ECT2, also known as epithelial cell transforming sequence 2 oncogene, was originally isolated as a transforming gene from epithelial cells.
ECT2 catalyzes guanine nucleotide exchange on the small GTPases, RhoA, Rac1, and Cdc42.
ECT2 may be phosphorylated during G2 and M phases, and phosphorylation may be required for its exchange activity.
Unlike other known guanine nucleotide exchange factors for Rho GTPases, ECT2 exhibits nuclear localization in interphase, spreads throughout the cytoplasm in prometaphase, and is condensed in the midbody during cytokinesis.
Expression of dominant-negative ECT2 or microinjection of affinity-purified ECT2 into interphase cells strongly inhibits cytokinesis.
These results suggest that ECT2 is an important link between the cell cycle machinery and Rho signaling pathways involved in the regulation of cell division.
Phosphorylation at T790 or S375 significantly affects the catalytic activity of ECT2.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:10,000-1:40,000Western Blot: 1:1000-1:4000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes human ECT2, phosphorylated at T790. Species Crossreactivity: mouse, rat, zebrafish, chimpanzee, chicken and canine.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Ca Ch Hu Mo Rb Rt Ze
Immunogen：Synthetic peptide corresponding to aa785-795 from human ECT2.
