The cytokine Interleukin 27(IL-27) is produced in response to inflammation.
It is made by activated presenting cells including monocytes, endothelial cells, and dendritic cells.
IL-27 consists of a heterodimeric combination of Epstein-Barr virus-induced molecule 3 (EBI3, or IL-27B) non-covalently linked with IL-27 p28 (or IL-27A).
It is a regulator of T helper cell development and suppressor of T-cell proliferation.
IL-27 has both pro and inflammatory properties.
It can stimulate cytotoxic T cell activity and induce isotype switching in B-cells.
It has diverse effects on innate immune cells.
It induces monocytes and mast cells to secrete pro-inflammatory cytokines.
When infection is present, IL-27 induces naive CD4+ T cells to proliferate and develop Th1 cell responses.
As an inflammatory regulator, IL-27 can inhibit Th1 or Th2 responses and restrict the strength and duration of adaptive immune responses.
The IL-27 p28 subunit, a 28kD glycoprotein belonging to the type I cytokine family, is homologous to IL-12 p35, IL-23 p19, and IL-6.
The EBI3 (Epstein-Barr virus-induced molecule 3, or IL-27B) subunit is a 34kD glycoprotein containing two fibronectin type III domains, and belongs to the type I cytokine receptor family.
It can exist as a homodimer and can also heterodimerize with IL-12 p35.
It is homologous to the p40 subunit of IL-12 and IL-23 and to the extracellular domain of IL-6 R.
EBI3 can heterodimerize also with IL-12 p35, or can exist as a homodimer.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:10,000-1:50,000Western Blot: 1:1000-1:5000Immunohistochemistry: 1:500-1:2500Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder is stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O prior to use.
To avoid repeated freezing and thawing, aliquot reconstituted stock solution and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Highly Purified
Purity：Purified by delipidation, salt fractionation and ion exchange chromatography.
Form：Supplied as a lyophilized powder in PBS, pH 7.2, 0.1% BSA, 0.01% gentamicin sulfate. Reconstitute with 100ul sterile dH2O. Labeled with horseradish peroxidase (HRP).
Specificity：Recognizes mouse EBI-3. Species Crossreactivity: rat. No crossreactivity with human EBI-3. Cross-reactivity with IL-27 from other sources has not been determined.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Mo Rt
Immunogen：Recombinant protein corresponding to mouse EBI-3.
