DNA dependent protein kinase (also called DNAPK, DNPK1, HYRC1, protein kinase DNA activated catalytic polypeptide, XRCC7 and p460) consists of the 460kD DNA PKcs and a heterodimeric regulatory complex comprised of p70Ku and p80Ku.
DNA PKcs is a nuclear protein serine/threonine kinase present in a wide variety of eukaryotic species.
DNA PKcs phosphorylates transcription factors, Sp1, Oct-1, p53 and SV40 large T.
DNA PKcs is involved in repairing double stranded DNA breaks.
At the onset of apoptosis, DNA PKcs is rapidly inactivated by cleavage of the catalytic subunit into smaller polypeptides.
Proteolysis of DNA PKcs is inhibited by the cysteine protease inhibitors iodoacetamide and N-ethylmaleimide.
Alternative splicing can occur for this protein to produce at least two isoforms.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:300,000Western Blot: 1:500-1:2000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A/G affinity chromatography.
Form：Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes human DNA PKcs. Species Crossreactivity: chimpanzee.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aaa2609 from human DNA PKcs.
