DIA-2 (also called DIA drome, Dia2, DIAPH2, Diaphanous 2, Diaphanous related formin 2, Diaphorase 2, DRF2, POF and POF2) may play a role in the development and normal function of the ovaries.
Mutations of this gene have been linked to premature ovarian failure.
Alternative splicing results in two protein isoforms.
Furthermore, each splice variant undergoes additional splicing in the 3' UTR.
Overall 4 splice variants have been described.
DIA-2 is expressed in testis, ovary, small intestine, prostate, lung, liver, kidney and leukocytes and can be found from E16 in ovary and testis and during P6-P16 during differentiation of ovarian follicles.
Isoform 1 is referred to as DIA-156, whereas isoform 2 is called DIA-12C.
Applications:Suitable for use in ELISA, Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:ELISA: 1:12,000-1:60,000Western Blot: 1:1000-1:6000Immunoprecipitation: 1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A/G affinity chromatography.
Form：Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes human DIA-2.
Isotype：IgG
Calc Applications Abbrev：E IP WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa1085-1101 from human DIA-2.
