Decorin-binding protein B, or DbpB, binds to decorin, which may mediate the adherence of B.burgdorferi to collagen fibers in skin and other tissues.
Spirochetal surface adhesions mediate attachment to decorin, a major component of the host extracellular matrix enabling bacteria to colonize in mammalian tissues.
The spirochete migrates from the tick midgut during feeding to its salivary glands and are thus transmitted to the mammal host.
This transition may be facilitated by changes in expression of some B.
burgdorferi genes.
It is believed that expression of the various proteins associated with the spirochete may be regulated by the changes in tick life cycle, changes in conditions during tick feeding (such as temperature, pH, and nutrients) and/or in coordination with the course of infection of the mammal host.
Borrelia burgdorferi can colonize multiple tissues, and is capable of attachment to diverse cell types.
The expression of decorin-binding protein (Dbp) A and/or DbpB, two B.
burgdorferi surface proteins that bind GAGs, is sufficient to convert a high-passage nonadherent B.
burgdorferi strain into one that efficiently binds 293 epithelial cells.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile dH2O or PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a lyophilized powder in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide. Reconstitute with sterile dH2O or PBS.
Specificity：Recognizes Borrelia burgdorferi DbpB.
Isotype：IgG
Calc Applications Abbrev：WB
Immunogen：MBP-fusion protein corresponding to Borrelia burgdorferi Dbp-B.
