Cyclin B1(also called CCNB1 and G2/mitotic-specific cyclin B1) is a regulatory protein involved in mitosis.
The gene product complexes with p34(cdc2) to form a serine/threonine kinase holoenzyme complex called the maturation-promoting factor (MPF).
Two alternative transcripts have been found a constitutively expressed transcript and a cell cycle-regulated transcript that is expressed predominantly during G2/M phase.
The different transcripts result from the use of alternate transcription initiation sites.
S126 phosphorylation, the site of phosphorylation recognized, is involved in an auto-phosphorylation of the cdk-cyclin complex.
Recognition of this phosphorylated site suggests is useful to detect the active form of the complex and may serve as a marker of mitosis.
Applications:Suitable for use in ELISA, Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:ELISA: 1:50,000Western Blot: 1:100-1:1000Immunoprecipitation: 1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes human Cyclin B1. Species Crossreactivity: rat, canine, chimpanzee, mouse and hamster.
Isotype：IgG
Calc Applications Abbrev：E IP WB
Calc Crossreactivity：Ca Hm Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa120-131 from human Cyclin B1.
