Cullins assemble a potentially large number of ubiquitin ligases by binding to the RING protein ROC1 to catalyse polyubiquitination, as well as binding to various specificity factors to recruit substrates.
Cullin 3 is an essential component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription.
In the SCF complex, cul3 serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits.
Cul3 may also contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme.
Unlike Cul1 and Cul2, Cul3 seems not to be a part of the SCF complex consisting of CUL1, RBX1, SKP1 and SKP2.
Cul3 also interacts with RNF7 and is part of a complex with TIP120A/CAND1, Cyclin E and RBX1.
Applications:Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:2000-1:10,000Western Blot: 1:500-1:1000Immunoprecipitation: 1:100, immunoprecipitates in vitro translated product and protein from cell lysates (using HeLa or NIH-3T3)Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid, 0.01% sodium azide.
Specificity：Recognizes human Cul3.
Isotype：IgG
Calc Applications Abbrev：E IHC IP WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa1-12 from human Cul3 at N-terminal, conjugated to KLH.
