Cullins assemble a potentially large number of ubiquitin ligases by binding to the RING protein ROC1 to catalyse polyubiquitination, as well as binding to various specificity factors to recruit substrates.
Cullin 5 is a component of E3 ubiquitin ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins.
Cullin 5 seems to be involved proteosomal degradation of p53/TP53 stimulated by adenovirus E1B-55kD protein.
Cullin 5 may form a cell surface vasopressin receptor.
Cullin 5 is part of a E3 ubiquitin ligase complex with elongin BC complex (TCEB1 and TCEB2), RBX1 and MUF1, complexes with elongin BC complex (TCEB1 and TCEB2), RXB1 and TCEB3 or SOCS1 or WSB1; elongin BC complex (TCEB1 and TCEB2), RBX1 and VHL; elongin BC complex (TCEB1 and TCEB2), RBX1, adenovirus type 5 E1B-55kD protein and adenovirus type 5 E4-orf6.
Cullin 5 Interacts with RBX1 and RNF7.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:2000-1:10,000 Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid, 0.01% sodium azide.
Specificity：Recognizes human Cul5.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa4-18 from human Cul5 at N-terminal, conjugated to KLH.
