CHK1(also known as serine/threonine-protein kinase CHK1 and checkpoint kinase 1) is involved in cell cycle arrest when DNA damage has occurred or when unligated DNA is present.
The kinase controls phosphorylation and abundance of PDS1 to prevent anaphase entry.
Also helps prevent mitotic exit.
CHK1 is localized within the nucleus.
Applications:Suitable for use in ELISA, Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:ELISA: 1:2000-1:10,000Western Blot: 1:200-1:2000Immunoprecipitation: 1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes Saccharomyces cerevisiae CHK1. Species Crossreactivity: yeast.
Isotype：IgG
Calc Applications Abbrev：E IP WB
Calc Crossreactivity：Ye
Immunogen：Synthetic peptide corresponding to aa312-327 from S.cerevisiae CHK1.
