84-2132-68　［取扱停止］FLIPa (CFLAR, CASP8 and FADD-like Apoptosis Regulator, FLIP) 100ug　168099

特徴

• Apoptosis is related to many diseases and is induced by a family of cell death receptors and their ligands.
• Cell death signals are transduced by death domain (DD)-containing adapter molecules and members of the ICE/CED-3 protease family.
• Caspases-8 (FLICE) and -10 (FLICE2) are two pivotal members in the ICE/CED-3 protease family.
• FLICE-inhibitory proteins were identified in virus and human and designated v-FLIPs and FLIP, respectively.
• The human FLIP was also cloned by several labs independently and termed Casper, I-FLICE, FLAME-1, CASH and CLARP.
• FLIP contains two death effector domains (DEDs) and a caspase-like domain.
• FLIP interacts with adapter protein FADD and caspase-8 and -10, and potently inhibits apoptosis induced by all known death receptors.
• Four splice variants of FLIPs have been identified and termed FLIPa, B, ?, and d, respectively.
• Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.5-1ug/mlImmunohistochemistry: 10ug/mlOptimal dilutions to be determined by the researcher.
• Positive Control:HeLa whole cell lysate
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.