84-2132-59　CDC20 (Cell Division Cycle Protein 20 Homolog, p55CDC) 100ul　168089

特徴

• CDC20, also known as fizzy, Cell division cycle protein 20 homolog, and p55CDC is required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex.
• CDC20 appears to act as a regulatory protein interacting with several other proteins at multiple points in the cell cycle.
• It is required for two microtubule-dependent processes, nuclear movement prior to anaphase and chromosome separation.
• CDC20 is regulated by MAD2L1.
• In metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates.
• The phosphorylated form of CDC20 interacts with APC/C.
• Synthesis is initiated at G1/S, protein level peaks in M phase and protein is abruptly degraded at M/G1 transition.
• Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:2000-1:10,000Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.