Cyclin T1, together with the kinase CDK9, is a component of the transcription elongation factor P-TEFb which binds the human immunodeficiency virus type 1 (HIV-1) transactivator Tat.
Tat stimulates human HIV-1 viral transcription elongation.
This suggests that cyclin T1/cdk9 (PITALRE) is one of the HIV-1 required host cellular cofactors generated during T cell activation.
Cyclin T1/cdk9 (PITALRE) is shown to interact with Tat to restore Tat activation in HeLa nuclear extracts depleted of P-TEFb.
P-TEFb facilitates transcription by phosphorylating the C-terminal domain (CTD) of RNA polymerase II.
The cdk9 (PITALRE) activity and cyclin T1 are essential for activation of transcription when tethered to the heterologous Rev response element RNA via the regulator of expression of virion Rev.
Cyclin T1 is an exceptionally large cyclin and is therefore a candidate for interactions with regulatory proteins.
Applications:Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:1000-1:5000Western Blot: 1:500-1:2000Immunohistochemistry: 1:200-1:1000Immunoprecipitation: 1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes human Cyclin T1. Species Crossreactivity: mouse and rat.
Isotype：IgG
Calc Applications Abbrev：E IHC IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to human Cyclin T1, conjugated to KLH.
