Chemokine (C-C motif) ligand 3-like 1 (CCL3L1), also known as macrophage inflammatory protein 1a(MIP-1a; other synonyms: ld78b, scya3l1, small inducible cytokine a3-like 1), is a member of the CC or beta chemokine subfamily that was originally purified from the conditioned media of an LPS-stimulated murine macrophage cell line.
In humans, CCL3L1 is encoded by a variable copy-number gene.
The CC family induces MCP1 and RANTES, and exhibits a variety of proinflammatory activities including chemotaxis, and functional and proliferative activation of leukocytes, lymphocytes, and macrophages.
Its signal is transmitted through transmembrane receptors, CC chemokine receptors, CCR1, CCR3 and CCR5.
CCL3L1/MIP-1 alpha acts as a chemoattractant to a variety of cells including monocytes, T cells, B cells and eosinophils.
CCL3L1 binds to several chemokine receptors.
In humans these receptors include chemokine binding protein 2 and chemokine (C-C motif) receptor 5 (CCR5).
CCR5 is a co-receptor for HIV, and binding of this protein to CCR5 inhibits HIV entry.
In swine, CCL3L1 protein is 86% similar to CCL4 protein, and 73% similar to CCL5.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile dH2O or PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：porcine
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a lyophilized powder in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide. Reconstitute with sterile dH2O or PBS.
Specificity：Recognizes porcine CCL3L1. Species Crossreactivity: bovine. Species sequence homology: feline, canine, human, chimpanzee, baboon, bovine, and rat (90%).
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Bo Po
Immunogen：Recombinant protein corresponding to 70aa from porcine CCL3L1, expressed in yeast.
