CCL2 is a small cytokine belonging to the CC chemokine family that recruits monocytes, memory T cells, and dendritic cells to sites of tissue injury and infection.
This chemokine is produced as a protein precursor containing a signal peptide of 23aa and a mature peptide of 76aa.
It is a monomeric polypeptide, with a total molecular weight of approximately 8.8kD.
The cell surface receptors that bind CCL2 are CCR2 and CCR4.
CCL2 is found at the site of tooth eruption and bone degradation.
In the bone, CCL2 is expressed by mature osteoclasts and osteoblasts and is under the control of nuclear factor kB (NFkB).
In human osteoclasts, CCL2 and RANTES (regulated on activation normal T cell expressed and secreted) may act as an autocrine loop in human osteoclast differentiation.
CCL2 causes the degranulation of basophils and mast cells, an effect potentiated by pre-treatment with IL-3 and other cytokines.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:4500Western Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile dH2O or PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：bovine
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a lyophilized powder in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide. Reconstitute with sterile dH2O or PBS.
Specificity：Recognizes bovine CCL2. Species Crossreactivity: porcine, human, feline, orangutan, canine and mouse.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Bo Ca Fe Hu Mo Po
Immunogen：Recombinant protein corresponding to 76aa from bovine CCL2, expressed in yeast.
