Three distinct signaling pathways lead to programmed cell death (apoptosis).
Two of these are the death receptor and mitochondrion pathways, in which the key apoptotic proteases caspase-8 and caspase-9, respectively, are involved.
Endoplasmic reticulum (ER) stress is the third apoptotic pathway and caspase-12 is involved (1,2).
Caspase-12 is localized to the ER but not to the cytoplasm or mitochondrion.
Caspase-12 is activated by ER stress, including disruption of ER calcium homeostasis, and mediates ER stress-induced apoptosis.
Caspase-12 is colocalized to the ER with several proteins that are involved in Alzheimer’s disease, including g-secretase presenilin and b-amyloid precursor protein (APP).
Caspase-12 mediates cytotoxicity induced by amyloid-b.
Caspase-12 is ubiquitously expressed in mouse tissues.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-1ug/mlImmunohistochemistry: 10ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes mouse Caspase-12 at ~48kD. Species Crossreactivity: human and rat.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids near the N-terminus of mouse Caspase-12.
