CaM Kinase IV (also known as CAM kinase-GR and CaMK IV) is a calcium/calmoduli dependent protein kinase belonging to a proposed calcium-triggered signaling cascade.
This kinase may be involved in the transcriptional regulation of microtubule dynamics.
In vitro, CaMK IV phosphorylates CREB1, CREBBP, PRM2, MEF2A, MEF2D and STMN1/OP18.
CaMK IV may also be involved in spermatogenesis and may play a role in the consolidation/retention of hippocampus-dependent long-term memory.
CaMK IV must be phosphorylated to be maximally active and is phosphorylated by CAMKK1 or CAMKK2.
In addition autophosphorylation of the N-terminal is required for full activation.
Autophosphorylation of 336aa allows the kinase to switch to a Ca(2+)/calmodulin-independent state.
Most likely the kinase is inactivated by the serine/threonine protein phosphatase 2A.
CaMK IV is a monomer that is located within the cytoplasm and nucleus and substantial localization occurs in certain neuronal nuclei.
In spermatids CaMK IV is associated with chromatin and the nuclear matrix.
CaMK IV is also specifically expressed in epithelial ovarian cancer tissue.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:5000-1:25,000Western Blot: 1:500-1:2000Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile dH2O or PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide. Reconstitute with sterile dH2O or PBS.
Specificity：Recognizes human CaM kinase IV. Species Crossreactivity: mouse, rat, canine, bovine and chimpanzee.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Bo Ca Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa305-323 from human CaM kinase IV.
