Epitope tags are short peptide sequences that are easily recognized by tag-specific.
Due to their small size, epitope tags do not affect the tagged protein's biochemical properties.
Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence.
This allows epitope tag to serve as universal detection reagents for any tag containing protein produced by recombinant means.
This means that epitope tags are a useful alternative to generating specific to identify, immunoprecipitate or immunoaffinity purify a recombinant protein.
The epitope tag is usually functional in a variety of dependent experimental procedures.
Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100,000-1:500,000Western Blot: 1:2000-1:10,000Immunohistochemistry: 1:1000-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile dH2O or PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Affinity Purified
Form：Supplied as a lyophilized powder in 0.02M PBS, pH 7.2, 0.01% sodium azide, 10mg/ml BSA. Reconstitute with sterile dH2O or PBS. Labeled with Biotin.
Specificity：Recognizes MYC Epitope Tag.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa410-419 from human c-Myc conjugated to KLH using maleimide.
