Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU) is a synthetic thymidine nucleoside analog.
BrdU is commonly used to allow the detection of growing or proliferating cells in living tissues.
During the S-phase of cell division, DNA replication occurs, and BrdU can be incorporated into the newly synthesized DNA by substituting for naturally occurring thymidine.
Specific for BrdU subsequently used to detect the incorporated BrdU thymidine analog.
This highlights cells that were actively replicating their DNA and is suggestive of actively growing cells.
Binding usually requires the DNA to be denatured, typically by exposing the cells to acid or heat.
Applications:Suitable for use in Immunofluorescence, ELISA, Western Blot, Flow Cytometry, Immunoprecipitation and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Immunofluorescence: 1:500ELISA: 1:2000-1:10,000Flow Cytometry: 1:50-1:100Immunohistochemistry: 1:100-1:500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide
