BORIS (Brother of the Regulator of Imprinted Sites) also known as CCCTC-binding factor-like protein, is normally only expressed in the testis and expressed in a mutually exclusive manner with CTCF during male germ cell development.
However, previous studies have shown that BORIS is abnormally activated in a wide range of human cancers.
Expression of BORIS in normally BORIS-negative cells promotes cell growth that may lead to transformation.
BORIS maps to the cancer-associated amplification region thought to contain an oncogene or dominant-immortalizing gene.
BORIS is a candidate protein for the epigenetic reprogramming factor acting in the male germ line.
BORIS is found in both the nucleus and cytoplasm.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:2000-1:10,000Western Blot: 1:200-1:2000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes human BORIS. Species Crossreactivity: chimpanzee.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa9-26 from human BORIS.
