Accumulation of the amyloid-b (Ab) plaque in the cerebral cortex is a critical event in the pathogenesis of Alzheimer’s disease.
Ab peptide is generated by proteolytic cleavage of the b-amyloid protein precursor (APP) at b- and g-sites by two proteases.
APP is first cleaved by b-secretase, producing a soluble derivative of the protein and a membrane anchored 99aa C-terminal fragment (C99).
The C99 fragment serves as the substrate for g-secretase to generate the 4kD amyloid-b peptide, which is deposited in the brains of all sufferers of Alzheimer’s disease.
The long-sought b-secretase was recently identified by several groups independently and designated B-site APP cleaving enzyme (BACE) and aspartyl protease 2 (Asp2) (1-4).
BACE/Asp2 is a novel transmembrane aspartic protease and colocalizes with APP.
Applications:Suitable for use in ELISA, Western Blot and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:10,000-1:50,000Western Blot: 0.5-1ug/mlImmunocytochemistry: 10ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Highly Purified
Purity：Purified by DEAE chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes human BACE at ~70kD. Species Crossreactivity: mouse and rat.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids near the C-terminus of human BACE.
