Aurora kinase B (also known as Aurora and Ipl1-like midbody-associated protein 1, AIM-1, Aurora/IPL1-related kinase 2, Aurora-related kinase 2, STK-1, and Aurora-B) is a Ser/Thr protein kinase member of the Aurora subfamily that may be directly involved in regulating the cleavage of polar spindle microtubules and is a key regulator for the onset of cytokinesis during mitosis.
Aurora kinase B is localized to the midzone of central spindle in late anaphase and concentrated into the midbody in telophase and cytokinesis and is colocalized with g-tubulin in the mid-body.
High levels of Aurora B expression are seen in the thymus, although it is also expressed in the spleen, lung, testis, colon, placenta and fetal liver.
Aurora B is expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase.
Applications:Suitable for use in Immunofluorescence, ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:10,000-1:50,000Western Blot: 1:250-1:2000Immunofluorescence: 1:50-1:500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A/G affinity chromatography.
Form：Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes human Aurora Kinase B.
Isotype：IgG
Calc Applications Abbrev：E IF WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa223-234 from human Aurora Kinase B.
