ATG13 is a target of the TOR kinase signaling pathway that regulates autophagy through the control of the phosphorylation status of ATG13 and ULK1 through their stable complex, and the regulation of ATG13-ULK1-RB1CC1.
ATG13 also forms a stable complex with FIP200.
Ulk1 phosphorylates ATG13 on S318 and promotes its release to damaged mitochondria.
Autophagy is a normal process in eukaryotes required for turnover of cellular components during starvation and stress.
It plays an essential role in cellular differentiation, cell death and aging.
Defects in this evolutionarily conserved process may contribute to certain human diseases such as cancer, neurodegenerative diseases, muscular disorders and pathogen infections.
ATG13 is one of several ATG genes required for autophagosome formation in mammalian cells.
mTOR interacts with this complex in a nutrient dependent manner and phosphorylates Atg13 and ULK1.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:220,000-1:250,000Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes human ATG13.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to the S318 region of ATG13.
