The ARF tumor suppressor is a critical regulator of p53 stability.
In addition to p53, ARF1 binds to other proteins such as MDM2 and ARF-BP1, a large protein containing HECT, UBA and WWE motifs.
ARF-BP1 directly binds and ubiquitinates p53; this activity is inhibited by ARF, indicating that ARF-BP1 is a critical mediator of the p53-dependent and p53-independent tumor suppressor functions of ARF.
ARF-BP1 can also catalyze the polyubiquitination of Mcl-1, an apoptotic Bcl-2 family member involved in DNA damage-induced apoptosis.
Elimination of ARF-BP1 expression by RNA interference stabilized Mcl-1 protein, resulting in an attenuation of apoptosis induced by DNA-damage agents.
Applications:Suitable for use in Immunofluorescence, Immunocytochemistry, ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 5ug/mlELISA: 1:10,000-1:40,000Western Blot: 1-2ug/ml, band at ~481kDOptimal dilutions to be determined by the researcher.
Positive Control:Daudi cell lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes human ARF-BP1. Species Crossreactivity: mouse and rat.
Isotype：IgG
Calc Applications Abbrev：E IC IF WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to a region near the C-terminus of human ARF-BP1.
