84-2132-05 AP2A (A-adaptin A, Adaptor protein complex AP-2 a-1 subunit, Clathrin assembly protein complex 2a-A large chain, 100kD coated vesicle protein A, plasma membrane adaptor HA2/AP2) 100ug 168026
84-2132-05 AP2A (A-adaptin A, Adaptor protein complex AP-2 a-1 subunit, Clathrin assembly protein complex 2a-A large chain, 100kD coated vesicle protein A, plasma membrane adaptor HA2/AP2) 100ug 168026
The AP2A1 and AP2A2 represent the AP2-a proteins that are found in the AP2 complex in clathrin-coated vesicles.
AP2A is also known as A-adaptin A, Adaptor protein complex AP-2 a-1 subunit, Clathrin assembly protein complex 2a-A large chain, 100kD coated vesicle protein A and plasma membrane adaptor HA2/AP2 adaptin a A subunit.
The AP2 complex is a heterotetramer consisting of two large adaptins (a or b), a medium adaptin (mu), and a small adaptin (sigma).
The complex is part of the protein coat on the cytoplasmic face of coated vesicles, which link clathrin to receptors in vesicles.
Applications:Suitable for use in ELISA, Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:ELISA: 1:5000-1:20,000Western Blot: 1:500-1:2000Immunoprecipitation: 1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:100ug
Host:goat
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.
Specificity:Recognizes human AP2A. Species Crossreactivity: chimpanzee, orangutan, mouse, rat, chicken and canine.
Isotype:IgG
Calc Applications Abbrev:E IP WB
Calc Crossreactivity:Ca Ch Hu Mo Rt
Immunogen:Synthetic peptide corresponding to aa3-14 from human AP2A1 and AP2A2.