APC1 (also known as anaphase promoting complex subunit 1, cyclosome subunit 1, protein Tsg24, mitotic checkpoint regulator and ANAPC1) is 1 of at least 11 subunits of the anaphase-promoting complex (APC), which functions at the metaphase-to-anaphase transition of the cell cycle and is regulated by spindle checkpoint proteins.
The APC is an E3 ubiquitin ligase that targets cell cycle regulatory proteins for degradation by the proteasome, thereby allowing progression through the cell cycle.
Applications:Suitable for use in ELISA, Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:ELISA: 1:10,000-1:120,000Western Blot: 1:200-1:2000Immunoprecipitation: 1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes human APC1. Species Crossreactivity: mouse, canine, rat and bovine.
Isotype：IgG
Calc Applications Abbrev：E IP WB
Calc Crossreactivity：Bo Ca Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa351-359 from human APC1.
