84-2131-94　AKT (Protein Kinase B, PKB, Rac, Related to A and C kinases) 100ug　168014

特徴

• AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at 473aa and 308aa.
• AKT is a cytoplasmic protein also known as Protein Kinase B (PKB) and rac (related to A and C kinases).
• AKT is a key regulator of many signal transduction pathways.
• AKT Exhibits tight control over cell proliferation and cell viability.
• Overexpression or inappropriate activation of AKT is noted in many types of cancer.
• AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin).
• PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation.
• Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus.
• AKT has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation.
• AKT is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.
• Applications:Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:5000-1:20,000Western Blot: 1:500-1:2000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.