Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation.
These changes are triggered by the activation of members of the caspase family, caspase activated DNase, and several novel proteins.
A novel gene, the product of which causes chromatin condensation and DNA fragmentation, was recently identified, cloned, and designated apoptosis inducing factor (AIF).
Like cytochrome c and caspase-9, which are critical molecules in apoptosis, AIF localizes to mitochondria.
AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9.
AIF induces chromatin condensation and large scale DNA fragmentation, which are the hallmarks of apoptosis.
These effects occur in both isolated nuclei and in the nuclei of live cells treated by microinjection and with apoptosis stimuli (2,3).
AIF is highly conserved between human and mouse and is widely expressed.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.25-1ug/mlImmunohistochemistry: 10ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:K562 cell lysate, as well as rat and mouse heart tissue lysates
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Highly Purified
Purity：Purified by DEAE chromatography from serum.
Form：Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes human AIF. Species Crossreactivity: mouse and rat.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids at an internal region of human AIF.
