84-2131-88 ACTB (Actin, Cytoplasmic 1, Beta-actin) (HRP) 100ug 168007
特徴
- In vertebrates 3 main groups of actin isoforms, A, B and G have been identified.
- The A-actins are found in muscle tissues and are a major constituent of the contractile apparatus.
- The B and G-actins coexist in most cell types as components of the cyto-skeleton and as mediators of internal cell motility.
- B-actins are highly conserved proteins that are involved in cell motility, structure and integrity.
- B-actins are cytoplasmic proteins.
- Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
- Other applications not tested.
- Recommended Dilution:ELISA: 1:20,000Western Blot: 1:1000-1:5000Immunohistochemistry: 1:500-1:2500Optimal dilutions to be determined by the researcher.
- Storage and Stability:Lyophilized powder may be stored at -20°C.
- Stable for 12 months at -20°C.
- Reconstitute with sterile dH2O or PBS.
- Aliquot to avoid repeated freezing and thawing.
- Store at -20°C.
- Reconstituted product is stable for 12 months at -20°C.
- For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Labeled with horseradish peroxidase (HRP).
- Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
- HRP conjugates are sensitive to light.
仕様
- Size:100ug
- Host:rabbit
- Source Antibody:human
- Grade:Affinity Purified
- Purity:Purified by immunoaffinity chromatography.
- Form:Supplied as a lyophilized powder in PBS, 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% gentamicin sulfate, 10mg/ml BSA. Reconstitute with sterile dH2O or PBS. Labeled with horseradish peroxidase (HRP).
- Specificity:Recognizes human B-actin. Species Crossreactivity: Mouse, rat, monkey, sheep, bovine, goat, guinea pig, porcine and hamster.
- Isotype:IgG
- Calc Applications Abbrev:E IHC WB
- Calc Crossreactivity:Bo Gt Gp Hm Hu Mk Mo Po Rt Sh
- Immunogen:Synthetic peptide corresponding to aa359-368 from human B-Actin.
- Shelf Life:1year
- EU Commodity Code:30021010
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