In vertebrates 3 main groups of actin isoforms, A, B and G have been identified.
The A-actins are found in muscle tissues and are a major constituent of the contractile apparatus.
The B and G-actins coexist in most cell types as components of the cyto-skeleton and as mediators of internal cell motility.
B-actins are highly conserved proteins that are involved in cell motility, structure and integrity.
B-actins are cytoplasmic proteins.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100,000Western Blot: 1:2000-1:10,000Immunohistochemistry: 1:1000-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile dH2O or PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 10mg/ml BSA, 0.01% sodium azide. Reconstitute with sterile dH2O or PBS. Labeled with Biotin.
Specificity：Recognizes human B-actin. Species Crossreactivity: mouse, rat, monkey, sheep, bovine, goat, guinea pig, porcine and hamster.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Bo Gt Gp Hm Hu Mk Mo Po Rt Sh
Immunogen：Synthetic peptide corresponding to aa359-368 from human B-actin.
