In vertebrates 3 main groups of actin isoforms, a, b and g have been identified.
A-actins are found in muscle tissues and are a major constituent of the contractile apparatus.
B and G-actins coexist in most cell types as components of the cyto-skeleton and as mediators of internal cell motility.
B-actins are highly conserved proteins that are involved in cell motility, structure and integrity.
B-actins are cytoplasmic proteins.
Actin is a loading control and is critical for the correct interpretation of your western blot.
B-Actin Loading Control is used to normalize the levels of protein detected by confirming that protein loading is uniform across the gel.
Applications:Suitable for use in Immunofluorescence, Immunohistochemistry, ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:500-1:2000ELISA: 1:10,000-1:40,000Western Blot: 1:1000-1:4000, band at ~42kDOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ug
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes human B-Actin. Species Crossreactivity: Mouse, rat, monkey, sheep, bovine, goat, guinea pig, porcine and hamster.
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Bo Gt Gp Hm Hu Mk Mo Po Rt Sh
Immunogen：Synthetic peptide corresponding to C-terminal region near aa350-375 of human beta Actin.
