84-2131-78　ST3GAL2 (ST3 beta-Galactoside alpha-2,3-Sialyltransferase 2) 100ug　167697

特徴

• Sialyltransferases add sialic acid to glycoproteins or glycosphingolipids and play important roles in many biological processes including immune recognition, pathogen infection, and cell adhesion (1).
• Similar to ST3GAL1 (2), ST3GAL2 is a type II membrane protein localized in the trans­Golgi network that can transfer sialic acid to the Galb1­3GalNAc structure to form NeuAca2­3Galb1­3GalNAc found in terminal carbohydrate groups of particular glycoproteins, oligosaccharides and glycolipids (3).
• Unlike the ubiquitously expressed ST3GAL1 (4), ST3GAL2 is mainly expressed in heart, liver, skeletal muscle and various lymphoid tissues but not in brain and kidney (3).
• ST3GAL2 is responsible for the synthesis of monosialosyl globopentaosylceramide (MSGb5), also known as stage­specific embryonic antigen­4 (SSEA4) (5), a cell surface marker for testicular germ cell carcinoma, renal cell carcinoma, and mesenchymal stem cells (6).
• The enzymatic activity of recombinant human ST3GAL2 was determined using a phosphatase­coupled glycosyltransferase assay (7).
• Over aa52­350 human ST3GAL2 share 94% aa sequence identity with mouse and rat.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.5ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.