84-2131-77　Sirtuin 1 (Silent Mating Type Information Regulation 2 Homolog, SIRT1, SIR2alpha, SIR2L1, SIR2-like Protein 1) [KO Validated] 100ug　167695

特徴

• SIRT1 (SIR2­like protein 1; also NAD­dependent protein deacetylase sirtuin­1 and hSIR2) is a class I member of the sirtuin family of enzymes.
• Although its predicted MW is 81kD, it runs anomalously at 110­120kD in SDS­PAGE.
• It is a widely expressed nuclear protein that participates in the deacetylation of multiple proteins, including p300, p53, LKB1 and histone H1.
• Functionally, this has the effect of promoting heterochromatin formation, cell survival and resistance to oxidative stress.
• Metabolically, SIRT1 induces insulin secretion, inhibits glycolysis and suppresses fatty acid synthesis.
• Human SIRT1 is 747aa in length.
• It possesses two NLS's (aa32­39 and 223­230), an NES (aa138­145), and a sertuin­type deacetylase domain (aa241­495) that contains an NAD and Zn binding motif.
• There are at least 12 utilized Ser/Thr phosphorylation sites, plus two nitrosylated Cys and one acetylated Ala.
• There are also four potential isoform variants.
• One is 95kD in size and shows a deletion of aa454­639, a second is 17kD in size and contains a 16aa substitution for aa149­747, and a third contains an alternative start site at Met296.
• SIRT1 is also known to undergo proteolysis by cathepsin B at Val533Ser534, generating a fourth, C­terminally truncated 75kD isoform.
• Full­length SIRT1 is suggested to form trimers, while the 17kD isoform appears to form dimers.
• Over aa2­747, human and mouse SIRT1 share 86%aa sequence identity.
• Applications:Suitable for use in Western Blot and Flow Cytometry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.2ug/mlFlow Cytometry: 0.25ug/10e6 cellsOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.