84-2131-75　NUDT5 (ADP-Sugar Pyrophosphatase, Nudix motif 5, YSA1, YSA1H) 100ug　167687

特徴

• ADP-Sugar Pyrophosphatase (NUDT­5, Nucleoside diphosphate-linked moiety X motif 5; also YSA1H and 8-oxo-dGDP phosphatase) is a member of the Nudix hydrolase family of proteins.
• Although the predicted MW of NUDT-5 is 24kD, it runs anomalously at 32-36kD in SDS-Page.
• NUDT-5 is a widely expressed intracellular homodimer that has at least two functions.
• First, it possesses pyrophosphatase activity that prevents nonenzymatic ribosylation of proteins, and second, it hydrolyzes 8-oxoGDP (plus 8-OH-dADP), thus removing an oxidized nucleoside from the nucleotide pool and its potential for incorporation into either DNA or RNA.
• Such incorporation likely results in transcriptional or translational errors.
• Human NUDT-5 is 219aa in length (SwissProt #Q9UKK9).
• It is contains an N-terminal substrate binding region (aa28-51), a Nudix hydrolase domain (aa57-197) and an extended C-terminal a-helix (aa211-219) NUDT-5 is acetylated on Met1, Lys42, Lys210, and Lys218, and phosphorylated on Tyr74.
• There are two isoform variants, one that shows a deletion of aa163-165, and another that contains a 13aa substitution for aa164-219.
• Full­length human and mouse NUDT-5 share 82%aa sequence identity.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.2ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.