LYVE-1 (Lymphatic vessel endothelial hyaluronic acid receptor 1; also CRSBP1) is a 58-64kD, monomeric, glycoprotein member of the Link protein superfamily of hyaluronbinding molecules.
It has limited expression, being found on the cell surface of lymphatic endothelial cells, endothelial cells of lymphoid sinuses, nodal stromal cells, and macrophages plus dendritic cells.
HA (hyaluronan) is a nonsulfated, freestanding, repeating disaccharide consisting of GlcA (glucuronic acid) in b-linkage with GlcNAc (N-acetylglucosamine).
It should not be confused with heparan, which is sulfated, protein-linked, and composed of repeating GlcA/IdoA and GlcNAc residues in both a-and b-linkages.
HA is ubiquitous and occupies space between collagen fibers.
It undergoes both normal, and pathology-induced turnover, and presumably does so by binding to LYVE1, CD44 and HARE on lymphatic endothelium.
Ultimately, HA is transported to the liver and nodes where it undergoes degradation.
This may be necessary as low MW HA is proinflammatory.
LYVE-1 is also a receptor for PDGFBB and VEGFA, and LYVE1 ligation apparently induces endothelial cell contraction with the opening of intercellular junctions.
Based on mouse, rat LYVE1 is synthesized as a 343 amino acid (aa) type I transmembrane protein that contains a very long signal sequence (aa1-52).
The extracellular region is likely to be 182aa in length (aa53-234) and contain one Link domain (aa62-210).
LYVE12 is likely maintained in a default "off mode" by undergoing sialylation, possibly at Thr83.
Over aa53259, rat LYVE1 shares 82% and 60% aa sequence identity with mouse and human LYVE1, respectively.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/mlImmunohistochemistry: 5-15ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, Trehalose. Reconstitute with sterile PBS.
Specificity：Recognizes rat LYVE-1. Species Crossreactivity: Recombinant mouse LYVE-1 50%, recombinant human LYVE1
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to aa53-259 from rat LYVE-1, NS0-derived (Accession# NP_001099756).
