PSMB8 (Proteasome Subunit beta type8; Also b5i, RING10/Y2 and LMP7) is a 2324kD member of the peptidase T1B family of molecules.
It is expressed both constitutively and inducibly by IFNg in a wide variety of cells, including immature dendritic cells, preadipocytes, CD4+ T cells and monocytes.
PSMB8 contributes to the 700kD, 20S proteasome catalytic complex, a dynamic intracellular structure that participates in ATPdependent proteolytic activity.
PSMB8 qualifies as a btype, i (immuno)type proteasome, meaning it both plays a chymotrypsinlike role in the turnover of proteins, and is found in cytokineresponsive cells.
The peptides generated through PSMB8 activity are used as immunogens by MHCI molecules.
PSMB8 activity is dependent upon the removal of the PSMB8 precursor prosequence, an action that exposes a critical internal Thr residue.
Human PSMB8 is synthesized as a 2829kD, 276aa proprecursor.
It contains a 72aa autocleavable propeptide plus a 204aa mature region.
The mature region shows no identifiable standard structural motifs.
There is one alternative splice form that shows a 45aa substitution for aa149.
This isoform does not appear to participate in formation of a proteosome.
Over aa73276, human PSMB8 shares 92%aa sequence identity with mouse PSMB8.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.2mg/ml.
Specificity：Recognizes human LMP7/PSMB8. Species sequence homology: Recombinant human PSMB5, 1%.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Recombinant corresponding to aa73276 from human LMP7/PSMB8 expressed in E.coli (P28062).
