Lin28B (Lineage protein 28 homolog B) is a 3537kD member of the Lin28 family of proteins.
Although there is an accompanying Lin28/28A molecule, Lin28 and Lin28B are not alternative splice forms but represent products of separate genes.
Lin28B is found in both the cytoplasm and nucleus of fetal hematopoietic stem and progenitor cells, embryonic liver and multiple tumor types.
It is considered a regulator of miRNA activity.
Active miRNAs arise from transcribed RNAs that are first cleaved by nuclear RNase III, next cleaved by cytoplasmic Dicer, and finally associate with Argonaute to generate a RISC RNA silencing complex.
Lin28A participates in the cytoplasmic uridylation of miRNAs on their 3’end, blocking Dicer processing and initiating RNA degradation.
Lin28B, by contrast, acts in the nucleus and blocks the initial RNase III cleavage step.
Human Lin28B is 250aa in length (SwissProt # Q6ZN17).
It contains one CSD/cold shock domain (aa29102) plus two CCHCtype Znfinger RNA bindingdomains (aa127144 and 149166).
Phosphorylation is known to occur on Ser105.
There is one reported alternate start site at Met71, and there is a potential 11aa substitution for aa 14.
Two isoforms of LIN28B with apparent MWs of 22kD and 37kD have been reported.
Over aa83250, human Lin28B shares 84%aa sequence identity with mouse Lin28B.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5ug/mlImmunohistochemistry: 5-15ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.2mg/ml.
Specificity：Recognizes human LIN28B. Species sequence homology: Recombinant human LIN28B and recombinant human LIN28B; 4%
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to aa83-250 from human LIN-28B expressed in E.coli (Q6ZN17).
