84-2131-70　Latent TGF-b bp2 (LTBP2, LTBP-2) 100ug　167672

特徴

• LTBP­2 (Latent TGF­b Binding Protein 2; also C14orf141) is a 220­250kD secreted glycoprotein member of the LTBP family of proteins.
• In SDS­PAGE and under reducing conditions, LTBP­2 runs at 220­250kD; under nonreducing conditions, LTBP­2 runs between 205­220kD.
• Although it is described as being a TGF­b binding protein, it does not possess the ability to bind covalently to TGF­b.
• Instead, it appears to bind to heparin sulfate moieties on syndecan­4 and perlecan, plus fibrillin­1 and fibulin­5, suggesting a role in both elastic fiber formation and the maintenance of basement membrane integrity.
• In addition, LTPB­2 is now known to form a complex with PC5/6A intracellularly, and upon secretion, dampen PC5 activation in the extracellular environment.
• Cells known to express LTBP­2 include lens and nonpigmented ciliary epithelium, fibroblasts, vascular smooth muscle and chondrocytes.
• Mature human LTBP­2 is 1786aa in length (aa36­1821).
• It possesses three heparin­binding regions (aa94­115, 232­249, and 344­354), plus 20 EGF­like domains (aa187­1818) that contain four interspersed TGF­b type binding segments.
• Over aa36­1821, human LTBP­2 shares 80%aa sequence identity with mouse LTBP­2.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.