84-2131-69　HTRA1 (High Temperature Requirement Protein-1, IGFBP5-protease, PRSS11) 100ug　167659

特徴

• HTRA1 is a member of the mammalian HTRA (high temperature requirement A) serine protease family.
• The E.
• coli HTRA homolog functions as a chaperone­protease and is essential for bacterial survival at temperatures above 42 °C (1).
• Among the four mammalian HTRA proteins, HTRA1, -3 and -4 are secreted while HTRA2 is localized in mitochondria.
• HTRA1 contains an N-terminal insulin­like growth factor binding protein (IGFBP) domain, a Kazal-type trypsin inhibitor motif, and C-terminal trypsin-like protease and PDZ domains (2).
• It is involved in several pathologies including Alzheimer’s disease (3), rheumatoid arthritis (4), osteoarthritis (5) and age-related macular degeneration (6), although the mechanisms by which HTRA1 exerts its effects are not clear.
• HTRA1 also has properties of a tumor suppressor protein, which makes it a target for cancer therapy (7).
• In addition HTRA1 is known to regulate the TGF-b signaling pathway and bone mineralization (8, 9).
• rhHTRA1 lacks the N-terminal IGFBP and Kazal­like domains, but retains the serine protease and PDZ domains.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.