IFI30 (Gammainterferoninducible protein IP30; also gammainterferon [IFNg] inducible lysosomal thiol reductase/GILT and Legumaturain) is a 2530kD member of the GILT family of proteins.
It is constitutively expressed in B cells and dendritic cells, and induced by IFNg in nonAPCs.
IFI30 is both intracellular and secreted as an inactive glycosylated proenzyme.
The glycosylation pattern contains a terminal phosphorylated mannose, which is recognized by cell surface mannose6 phosphate receptors and internalized into lysosomes.
In lysosomes, IFI30 is processed into an active, mature form, and via a thiol reductase domain, breaks disulfide bonds in molecules destined for lysosomal degradation.
This is a critical first step in the processing and subsequent presentation of peptides that will initiate an antigenic response.
The human IFI30 proenzyme is 224aa in length.
In this form, it is 3335kD in size.
Following proteolytic processing at both the N and Cterminus, a 175aa, 2530kD active mature form is generated (aa58232).
The mature region possesses a thiol reductase domain (aa62151) plus one utilized Thr phosphorylation site.
Both the pro and mature forms exhibit enzymatic activity.
IFI30 is known to exist as a 5060kD disulfidelinked homodimer.
There are four potential isoform variants.
One contains a 26aa substitution for aa213250, a second shows a deletion of aa131161, a third shows a deletion of aa106123, while a fourth shows a deletion of aa64212.
Over aa27250, human IFI30 shares 62%aa sequence identity with mouse IFI30.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.2mg/ml.
Specificity：Recognizes human GILT/IFI30.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Recombinant corresponding to aa27250 from human GILT/IFI30 expressed in HEK293 cell (P13284).
