Sox9 is a transcription factor related to campomelic dysplasia and sex reversal in human patients.
Earlier studies in zebrafish led to the identification of two homologues of mammalian sox9, sox9a and sox9b.
The present study represents the first evaluation of expression patterns of zebrafish sox9b during early embryogenesis.
Analyses reveal that sox9b transcripts are present throughout the life-cycle of the zebrafish, but exhibit tissue-specific distribution during embryogenesis.
Zygotic expression of sox9b occurs in the anterolateral margins and the midline of the prospective dorsal neuroectoderm during late gastrulation.
During early segmentation, the transcript is expressed in pairs of longitudinal bands in the prospective midbrain, hindbrain, and trunk, which identify the cranial and trunk neural crest progenitors.
Neural crest cells cease expression of sox9b during migration, but some of their derivatives resume sox9b expression.
Sox9b can serve as a marker for neural crest precursors.2
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:64,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：Zebrafish
Grade：Purified
Purity：Purified by ammonium sulfate precipitation.
Form：Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Specificity：Recognizes Sox9b (aa248-259). Species Sequence Homology: Zebrafish
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Ze
Immunogen：Peptide with sequence C-PLQDGIDFGAVD, from the internal region of the protein sequence (NP_571719.1).
