FBL is a component of a nucleolar small nuclear ribonucleoprotein (snRNP) particle thought to participate in the first step in processing preribosomal RNA.
It is associated with the U3, U8, and U13 small nuclear RNAs and is located in the dense fibrillar component (DFC) of the nucleolus.
This protein contains an N-terminal repetitive domain that is rich in glycine and arginine residues, like fibrillarins in other species.
Its central region resembles an RNA-binding domain and contains an RNP consensus sequence.
Antisera from approximately 8% of humans with the autoimmune disease scleroderma recognize fibrillarin.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:128,000Western Blot: 0.1-0.3ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Purified
Purity：Purified by ammonium sulfate precipitation.
Form：Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Specificity：Recognizes human Fibrillarin/FBL (aa99-110). Species sequence homology: Mouse, rat, canine, porcine, bovine.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo
Immunogen：Peptide with sequence CRGKEDALVTKN, from the internal region of the protein sequence (NP_001427.2).
