CHK2, a protein kinase that is activated in response to DNA damage, is involved in cell cycle arrest.
Mapped on 22q12.1, CHK2 has a potential regulatory region rich in SQ and TQ amino acid pairs.
It regulates BRCA1 function after DNA damage by phosphorylating serine-988 of BRCA1.
Additionally, CHK2 can be modified by phosphorylation and activated in response to ionizing radiation, and can be also modified in response to hydroxyurea treatment.
Furthermore, oligomerization of CHEK2 increases the efficiency of transautophosphorylation, resulting in the release of active CHEK2 monomers that proceed to enforce checkpoint control in irradiated cells.
Morever, CHK2 is a tumor suppressor gene conferring predisposition to sarcoma, breast cancer, and brain tumors, and that their observations provided a link between the central role of p53 inactivation in human cancer and the well-defined G2 checkpoint in yeast.
There is a wide expression of small amounts of CHK2 mRNA with larger amounts in human testis, spleen, colon, and peripheral blood leukocytes.
pplications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilutions:ELISA: 1:64,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：drosophila
Grade：Affinity Purified
Purity：Purified by ammonium sulphate precipitation followed by affinity chromatography
Form：Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Specificity：Recognizes Drosophila Chk2.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Dr
Immunogen：Synthetic peptide corresponding toaa115-128, C-QDLSRNGTFVNNEK of Drosophila Chk2, at the internal region of the protein.
