Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor.
Recognizes the substrate consensus sequence [ST]-Q.
Phosphorylates various proteins, which collectively inhibits DNA replication and mitosis and promotes DNA repair and recombination.
Phosphorylates grp/CHK1.
Phosphorylates 'Ser-137' of histone variant H2AX/H2AV at sites of DNA damage, thereby regulating DNA damage response mechanism.
Essential for the DNA damage checkpoint in larval imaginal disks and neuroblasts and for the DNA replication checkpoint in the embryo.
Has also an essential role during early nuclear divisions in embryos, where it is required to delay mitosis in response to incomplete DNA replication.
Also plays an important role during meiosis, where it may monitor double-strand-break repair during meiotic crossing over, to regulate the progression of prophase I, and to enforce metaphase I delay observed at the end of oogenesis.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:128,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：drosophila
Grade：Purified
Purity：Purified by ammonium sulfate precipitation.
Form：Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Specificity：Recognizes Mei-41. Species Sequence Homology: drosophila
Isotype：IgG
Calc Applications Abbrev：E WB
Immunogen：Peptide with sequence DPHCAIEDIKVPLEQ, from the internal region of the protein sequence (NP_523369.2).
