Mitogen-activated protein kinase 1, also known as MAPK1, p42MAPK, and ERK2, is an enzyme which in humans is encoded by the MAPK1 gene.1 The gene is mapped to 22q11.2.
And the protein encoded by this gene is a member of the MAP kinase family.
The deduced 360aa human ERK2 protein shares 98% identity with rat Erk2.
The MAP kinase ERK2 is widely involved in eukaryotic signal transduction.
Upon activation, it translocates to the nucleus of the stimulated cell, where it phosphorylates nuclear targets.
Futhermore, the ERK pathway is necessary for experience-dependent plasticity and for long-term potentiation of synaptic transmission in the developing visual cortex.
And the MAPK pathway is also involved in responses to NTN1.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:8000Western Blot: 0.5-2ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:HepG2 Cell Lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Specificity：Recognizes human ERK2.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to 13aa from near the internal region of ERK2. Species sequence homology: mouse, rat, canine and bovine.
