Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies.
Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens.
Members of this subfamily include caspase-1, -4, -5, and -12.
Activation of these caspases results in the cleavage and activation of proinflammatory cytokines such as IL-1b and IL-18.
Caspase-4 was initially identified as a homologous protein to caspase-1 and the C.
elegans Ced-3, which could induce apoptosis in transfected cells.
More recent studies have shown that it can be activated by ER stress and has been suggested to be involved in multiple neuronal pathologies such as Alzheimer's disease.
This antibody is expected to recognize all 3 isoforms of CASP4 (alpha, represented by NP_001216; gamma, NP-150649 and delta NP_150650).
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:16,000Western Blot: 0.3-1ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Human Heart Tissue Lysate and Human Brain Tissue Lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Specificity：Recognizes human CASP4.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to 15aa from near the internal region of CASP4. Species sequence homology: mouse, feline, canine and bovine.
