COX proteins are membrane-associated heme proteins that have cyclooxygenase and peroxidase activities.
These enzymes are targets of NSAID (Nonsteroidal anti-inflammatory drugs) such as aspirin.
Prostaglandins (PGs) formed by the enzymatic activity of COX-1 are primarily involved in the regulation of homeostatic functions throughout the body, whereas PGs formed by COX-2 primarily mediate pain, fever, and inflammation.
COX-1 is constitutively expressed, with particularly high expression in gastrointestinal tissues.
COX-2 is induced by cytokines and mitogens and is likely to play a role in inflammatory diseases such as rheumatoid arthritis.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:32,000Western Blot: 1-3ug/mlImmunohistochemistry (Formalin fixed paraffin embedded): 3-5ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:HeLa Cell Lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Specificity：Recognizes human COX-1.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to 14aa from near the C-terminus of COX-1.
