This antibody is expected to recognize all reported isoforms (NP_001005743.1, 001005744.1, NP_003735, NP_001005745.1).
It is also likely to cross-react with Numblike (NUMBL, GeneID: 9253) which has a very similar C-Terminus, although this has not yet been tested experimentally.
Applications:Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:32,000Western Blot: 0.03-0.1ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Mouse Brain Tissue Lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:100ug
Host:goat
Source Antibody:mouse
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Specificity:Recognizes mouse NUMB.
Isotype:IgG
Calc Applications Abbrev:E IC IF WB
Calc Crossreactivity:Mo
Immunogen:Synthetic peptide corresponding to 14aa from near the C-terminus of NUMB. Species sequence homology: human, canine and bovine.