Defensins are a large family of broad-spectrum antimicrobial peptides, identified originally in leukocytes of rabbits and humans.
The genes encoding alpha human beta and defensins are clustered in a contiguous segment of chromosome 8p23.
Defensins are initially synthesized as inactive prodefensins with a signal peptide that is cleaved off by Matrilysin/MMP7 (a tissue metalloproteinase) to generate mature and bioactive defensin peptide.
Matrilysin is expressed in Paneth cell granules together with perhaps more than 20 different -defensins (cryptidins).
Disruption of the matrilysin gene prevents the normal posttranslational proteolytic activation of intestinal prodefensins.
Matrix metalloproteinase-7 (MMP-7) also known as matrilysin and PUMP (EC 3.4.24.23) cleaves a number of substrates including collagen types IV and X, elastin, fibronectin, gelatin, laminin and proteoglycans.
MMP-7 is closely related to the stromelysin family members but is encoded by a different gene.
MMP-7 is the smallest of all the MMPs consisting of a pro-peptide domain and a catalytic domain.
It lacks the hemopexin-like domain common to other members of the MMPs.
MMP-7 is secreted as a 28kD proenzyme and can be activated in vitro by organomercurials and trypsin and in vivo by MMP-3 to a 18kD active MMP-7 enzyme.
Once activated, MMP-7 can activate pro-MMP-1 and pro-MMP-9 but not pro-MMP-2.
MMP-7 is widely expressed having been reported in elevated levels in cycling endometrium as well as in colorectal cancers and adenomas, hepatocellular carcinomas, rectal carcinomas, and approximately 50% of gliomas.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:64,000Western Blot: 0.03-0.1ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Human Breast Tissue Lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Specificity：Recognizes human MMP-7.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to 13aa from near the C-terminus of MMP-7.
