HPK1, a KHS protein kinase, is an upstream activator of the stress-activated protein kinase (SAPK) signaling pathway.
The protein has a structure similar to other GCK (Group I) kinases and contains four consensus SH3 binding motifs, which have been shown to interact with the SH3 motifs of the MAP kinase kinase kinases MLK3 and MEKK1 and several adapter proteins that couple to tyrosine kinases, including BASH, Blnk, Clnk, Crk, CrkL and Grb2.
Additionally, HPK1 has been shown to be phosphorylated and activated by the tyrosine kinase c-Abl in response to genotoxic agents.
At least two mRNA transcripts have been reported.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:32,000Western Blot: 0.5-2ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Jurkat Cell Lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Specificity：Recognizes human MAP4K1.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to 13aa from near the N-terminus of MAP4K1. Species sequence homology: bovine, canine, mouse, porcine and rat.
