Fyn is a member of the Src family of protein tyrosine kinases.
Fyn has been shown to be involved in T cell and B cell activation as well as keratinocyte differentiation.
Fyn can regulate the activation of PI-3 kinase in CTLL-2 cells, a murine interleukin 2 responsive cell line.
In T cells, Fyn associates with the T cell antigen receptor and Thy-1.
A study of human T-cells revealed that Lck and Fyn had different intracellular locations, suggesting that these two members of the Src family regulate different aspects of T cell activation.
The Src homology 3 (SH3) region of Fyn can bind the proline-rich domain of the Fas ligand.
In addition, Raf-1 may form a complex with Fyn through its Src Homology 2 (SH2) domain and serine phosphorylation of Raf-1.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:32,000Western Blot: 0.2-1ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Jurkat Cell Lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Specificity：Recognizes human FYN. Species Crossreactivity: mouse.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to 13aa from near the N-terminus of FYN. Species sequence homology: rat, canine, porcine and bovine.
